Difference between revisions of "Part:BBa K1993021"
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Human ferritin heavy chain protein (hFTH) is a ferroxidase enzyme that in humans is encoded by the FTH1 gene. It is composed of 24 subunits of the heavy and light ferritin chains. A major function of ferritin is the storage of iron in a soluble and nontoxic state. What’s more, it could be detected by Magnetic Resonance Imaging (MRI) in separate studies for molecular imaging studies, providing an in vivo proof-of-concept for drug discovery projects in preclinical phase. [1] | Human ferritin heavy chain protein (hFTH) is a ferroxidase enzyme that in humans is encoded by the FTH1 gene. It is composed of 24 subunits of the heavy and light ferritin chains. A major function of ferritin is the storage of iron in a soluble and nontoxic state. What’s more, it could be detected by Magnetic Resonance Imaging (MRI) in separate studies for molecular imaging studies, providing an in vivo proof-of-concept for drug discovery projects in preclinical phase. [1] | ||
| − | In our project, in order to replace eGFP and dTomato for preclinical application and ensure our engineered MSCs were locating in inflamed lesions, we introduced hFTH gene. Firstly, we acquired it from a synthetic constructed plasmid we purchased from Addgene. (Figure 1) Secondly, we combined hFTH with other genes by Gateway technology and constructed a composite part, Luciferase-T2A-dTomato-T2A-hFTH[https://parts.igem.org/Part:BBa_K1993006 BBa_K1993006]. Thirdly, we transduced this composite part into MSCs. | + | In our project, in order to replace eGFP and dTomato for preclinical application and ensure our engineered MSCs were locating in inflamed lesions, we introduced hFTH gene. Firstly, we acquired it from a synthetic constructed plasmid we purchased from Addgene. (Figure 1) Secondly, we combined hFTH with other genes by Gateway technology and constructed a composite part, Luciferase-T2A-dTomato-T2A-hFTH [https://parts.igem.org/Part:BBa_K1993006 BBa_K1993006]. Thirdly, we transduced this composite part into MSCs. |
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Latest revision as of 12:50, 18 October 2016
hFTH
Human ferritin heavy chain protein (hFTH) is a ferroxidase enzyme that in humans is encoded by the FTH1 gene. It is composed of 24 subunits of the heavy and light ferritin chains. A major function of ferritin is the storage of iron in a soluble and nontoxic state. What’s more, it could be detected by Magnetic Resonance Imaging (MRI) in separate studies for molecular imaging studies, providing an in vivo proof-of-concept for drug discovery projects in preclinical phase. [1]
In our project, in order to replace eGFP and dTomato for preclinical application and ensure our engineered MSCs were locating in inflamed lesions, we introduced hFTH gene. Firstly, we acquired it from a synthetic constructed plasmid we purchased from Addgene. (Figure 1) Secondly, we combined hFTH with other genes by Gateway technology and constructed a composite part, Luciferase-T2A-dTomato-T2A-hFTH BBa_K1993006. Thirdly, we transduced this composite part into MSCs.
Figure 1 DNA of hFTH was shown in agarose gel electrophoresis.
Reference
[1] Rossi M, Massai L, Diamanti D, et al. Multimodal molecular imaging system for pathway-specific reporter gene expression. [J]. European Journal of Pharmaceutical Sciences Official Journal of the European Federation for Pharmaceutical Sciences, 2016, 86:136-142.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]