Difference between revisions of "Part:BBa K2074032"
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Cry10Aa and Cyt2 are protein from Bacillus thuringiensis (Bt),which have toxicity to the larave of mosquito.We used 2A system to express two protein at the same time in Chlamydomonas reinhardti. These two proteins express at the same time can enhance the toxicity to the larvae of mosquito. | Cry10Aa and Cyt2 are protein from Bacillus thuringiensis (Bt),which have toxicity to the larave of mosquito.We used 2A system to express two protein at the same time in Chlamydomonas reinhardti. These two proteins express at the same time can enhance the toxicity to the larvae of mosquito. | ||
[[File:2016FAFUCHINADevice5.png|800px|thumb|center|'''Fig.1''' Cry10Aa and Cyt2 Toxins Group]] | [[File:2016FAFUCHINADevice5.png|800px|thumb|center|'''Fig.1''' Cry10Aa and Cyt2 Toxins Group]] | ||
Revision as of 09:05, 18 October 2016
Cry11Aa(Codon optimization)+Extended FMDV 2A+Cyt2(Codon optimization)
Cry10Aa and Cyt2 are protein from Bacillus thuringiensis (Bt),which have toxicity to the larave of mosquito.We used 2A system to express two protein at the same time in Chlamydomonas reinhardti. These two proteins express at the same time can enhance the toxicity to the larvae of mosquito.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2195
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 517
Illegal NgoMIV site found at 1681
Illegal NgoMIV site found at 2029
Illegal NgoMIV site found at 2329 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 49
Illegal BsaI.rc site found at 244
Illegal BsaI.rc site found at 1459
Illegal SapI.rc site found at 1440