Difference between revisions of "Part:BBa K2074030"

Revision as of 09:04, 18 October 2016

Cry10Aa(Codon optimization)+Extended FMDV 2A+Cyt2(Codon optimization) Cry10Aa and Cyt2 are proteins from Bacillus thuringiensis (Bt),which have toxicity to the larvae of mosquito.We used 2A system to express two proteins at the same time in Chlamydomonas reinhardti. These two proteins express at the same time can enhance the toxicity to the larvae of mosquito.

Fig.1 Cry10Aa and Cyt2 Toxins Group

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 368
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 32
Illegal BglII site found at 2282
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 181
Illegal NgoMIV site found at 223
Illegal NgoMIV site found at 280
Illegal NgoMIV site found at 784
Illegal NgoMIV site found at 1460
Illegal NgoMIV site found at 2116
Illegal NgoMIV site found at 2416
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 202
Illegal SapI.rc site found at 924

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2074030 short</partinfo>
+https://static.igem.org/mediawiki/igem.org/thumb/9/9f/T--FAFU-CHINA--partsclones.png/800px-T--FAFU-CHINA--partsclones.png
 Cry10Aa and Cyt2 are proteins from Bacillus thuringiensis (Bt),which have toxicity to the larvae of mosquito.We used 2A system to express two proteins at the same time in Chlamydomonas reinhardti. These two proteins express at the same time can enhance the  toxicity to the larvae of mosquito.
 [[File:2016FAFUCHINADevice5.png|800px|thumb|center|'''Fig.1''' Cry10Aa and Cyt2 Toxins Group]]