Difference between revisions of "Part:BBa K2100040"
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<partinfo>BBa_K2100040 short</partinfo> | <partinfo>BBa_K2100040 short</partinfo> | ||
− | This | + | This construct is the result of a gateway combining a TALER14 repressible promoter upstream of the eYFP gene, a yellow fluorescent protein. pTALER14 consists of one repressible site upstream and four repressible sites downstream of the minimal CMV promoter. A VP-16 Gal4 protein complex recruits the transcription machinery of the promoter. The TALER14 repressor inhibits the activator and transcription machinery from transcribing the eYFP gene. |
Latest revision as of 04:59, 19 October 2016
pEXPR pTALER14 - eYFP
This construct is the result of a gateway combining a TALER14 repressible promoter upstream of the eYFP gene, a yellow fluorescent protein. pTALER14 consists of one repressible site upstream and four repressible sites downstream of the minimal CMV promoter. A VP-16 Gal4 protein complex recruits the transcription machinery of the promoter. The TALER14 repressor inhibits the activator and transcription machinery from transcribing the eYFP gene.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal XbaI site found at 157
Illegal PstI site found at 574 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal PstI site found at 574 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal BamHI site found at 303
Illegal XhoI site found at 246 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal XbaI site found at 157
Illegal PstI site found at 574 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal XbaI site found at 157
Illegal PstI site found at 574
Illegal AgeI site found at 357 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 318