Difference between revisions of "Part:BBa K2100010:Experience"
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We characterized our hybrid promoter in both MCF-7 and tHESC cells. The following characterization experiment in tHESC cells tested how the hybrid promoter responds to a 1 uM induction of progesterone (MPA). The cells were transfected with 250 ng of hEF1a-mKate as a transfection marker and 250 ng of pHybrid-eYFP for characterization. | We characterized our hybrid promoter in both MCF-7 and tHESC cells. The following characterization experiment in tHESC cells tested how the hybrid promoter responds to a 1 uM induction of progesterone (MPA). The cells were transfected with 250 ng of hEF1a-mKate as a transfection marker and 250 ng of pHybrid-eYFP for characterization. | ||
| − | + | https://static.igem.org/mediawiki/2016/1/17/T--MIT--hybridthesc.png | |
The next characterization experiment tested how the hybrid promoter responds to a 10 nM induction of E2 (estradiol) in MCF-7 cells. The cells were transfected with 250 ng of hEF1a-mKate as a transfection marker and 250 ng of pHybrid-eYFP for characterization. | The next characterization experiment tested how the hybrid promoter responds to a 10 nM induction of E2 (estradiol) in MCF-7 cells. The cells were transfected with 250 ng of hEF1a-mKate as a transfection marker and 250 ng of pHybrid-eYFP for characterization. | ||
| − | + | https://static.igem.org/mediawiki/2016/2/26/T--MIT--hybridmcf7.png | |
The results showed an 100 fold difference between the yellow fluorescent output of the uninduced tHESC cells and the ones induced with 1 uM MPA. | The results showed an 100 fold difference between the yellow fluorescent output of the uninduced tHESC cells and the ones induced with 1 uM MPA. | ||
Revision as of 00:01, 18 October 2016
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We characterized our hybrid promoter in both MCF-7 and tHESC cells. The following characterization experiment in tHESC cells tested how the hybrid promoter responds to a 1 uM induction of progesterone (MPA). The cells were transfected with 250 ng of hEF1a-mKate as a transfection marker and 250 ng of pHybrid-eYFP for characterization.
The next characterization experiment tested how the hybrid promoter responds to a 10 nM induction of E2 (estradiol) in MCF-7 cells. The cells were transfected with 250 ng of hEF1a-mKate as a transfection marker and 250 ng of pHybrid-eYFP for characterization.
The results showed an 100 fold difference between the yellow fluorescent output of the uninduced tHESC cells and the ones induced with 1 uM MPA.
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