Difference between revisions of "Part:BBa K2100004:Experience"
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===Applications of BBa_K2100004=== | ===Applications of BBa_K2100004=== | ||
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| − | ( | + | We characterized our TRE promoter induced with a fine sweep of Doxycycline from a concentration of 0 uM to 2000 uM. We transfected HEK293 cells with (250?)ng of TRE-mKate and 250 ng hEF1a-eYFP(?). We analyzed fluorescent output in the PE Texas Red channel to measure the activity of the TRE promoter under different DOX concentrations. We used 0 uM of DOX as a control. |
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| + | https://static.igem.org/mediawiki/parts/9/96/T--MIT--TRE.png | ||
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| + | The results indicate the optimal DOX concentration was 500 uM for maximum TRE activity. Beyond that point, adding a higher concentration of DOX does not significantly increase fluorescent output from the TRE-mKate construct. | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 12:26, 19 October 2016
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K2100004
We characterized our TRE promoter induced with a fine sweep of Doxycycline from a concentration of 0 uM to 2000 uM. We transfected HEK293 cells with (250?)ng of TRE-mKate and 250 ng hEF1a-eYFP(?). We analyzed fluorescent output in the PE Texas Red channel to measure the activity of the TRE promoter under different DOX concentrations. We used 0 uM of DOX as a control.
The results indicate the optimal DOX concentration was 500 uM for maximum TRE activity. Beyond that point, adding a higher concentration of DOX does not significantly increase fluorescent output from the TRE-mKate construct.
User Reviews
UNIQ64f5e322957d549c-partinfo-00000000-QINU UNIQ64f5e322957d549c-partinfo-00000001-QINU