Difference between revisions of "Part:BBa K1930006"
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<html> <a> <img src="https://static.igem.org/mediawiki/parts/6/61/T--Groningen--sfgfp-microscopy.jpeg" height=400"/></a></html> | <html> <a> <img src="https://static.igem.org/mediawiki/parts/6/61/T--Groningen--sfgfp-microscopy.jpeg" height=400"/></a></html> | ||
+ | Images were taken with an Olympus IX71 microscope (Personal DV, Applied Precision; assembled by Imsol, Preston, United Kingdom) using CoolSNAP HQ2 camera (Princeton Instruments, Trenton, NJ, USA) with a 100× phase-contrast objective. Fluorescence filter sets (excitation, 450 to 490 nm; emission, 500 to 550 nm) used to visualize GFP were from Chroma Technology Corporation (Bellows Falls, VT, USA). The exposure times were 0.2 s with 32% excitation xenon light (300 W) for B. subtilis. | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 23:31, 18 October 2016
sfGFP(Sp)
sfGFP(Sp) is a reporter gene originally optimized for Streptococcus pneumoniae.
It has been shown when expressed in B. subtilis that the signal is even brighter than the one from sfGFP(Bs). [http://aem.asm.org/content/79/20/6481.full]
Usage and Biology
Images were taken with an Olympus IX71 microscope (Personal DV, Applied Precision; assembled by Imsol, Preston, United Kingdom) using CoolSNAP HQ2 camera (Princeton Instruments, Trenton, NJ, USA) with a 100× phase-contrast objective. Fluorescence filter sets (excitation, 450 to 490 nm; emission, 500 to 550 nm) used to visualize GFP were from Chroma Technology Corporation (Bellows Falls, VT, USA). The exposure times were 0.2 s with 32% excitation xenon light (300 W) for B. subtilis.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]