Difference between revisions of "Part:BBa K2100036:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | This composite part expression vector was created by an LR reaction. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts. | |
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===Source=== | ===Source=== |
Revision as of 23:17, 17 October 2016
pEXPR TRE-BM3R1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 580
Illegal XbaI site found at 30 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 580
Illegal NheI site found at 453
Illegal NotI site found at 342 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 580 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 580
Illegal XbaI site found at 30 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 580
Illegal XbaI site found at 30 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 446
Illegal BsaI site found at 573
Design Notes
This composite part expression vector was created by an LR reaction. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
Source
Part comes from some genomic sequence.