Difference between revisions of "Part:BBa K1886006:Design"

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===References===
 
===References===
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Levskaya, A. et al (2005). Engineering Escherichia coli to see light. Nature, 438(7067), 442.
 
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Olson E J, Hartsough L A, Landry B P, et al. Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals[J]. Nature methods, 2014, 11(4): 449-455.
 
Olson E J, Hartsough L A, Landry B P, et al. Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals[J]. Nature methods, 2014, 11(4): 449-455.
 
MLA
 
MLA

Revision as of 22:51, 17 October 2016


broken Ptet-cph8


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2332
    Illegal XhoI site found at 439
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There was a SpeI site in the sequence encoding protein cph8, so we designed a silent mutation.



Source

From E coli genome, obtained from iGEM team HZAU's lab. This plasmid is bought from Addgene Company.

References

Olson E J, Hartsough L A, Landry B P, et al. Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals[J]. Nature methods, 2014, 11(4): 449-455. MLA