Difference between revisions of "Part:BBa K1886006:Design"

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===Source===
 
===Source===
  
−
From E coli genome, obtained from iGEM team HZAU's lab
+
From E coli genome, obtained from iGEM team HZAU's lab.
 
This plasmid is bought from Addgene Company.
 
This plasmid is bought from Addgene Company.
  

Revision as of 22:50, 17 October 2016


broken Ptet-cph8


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2332
    Illegal XhoI site found at 439
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There was a SpeI site in the sequence encoding protein cph8, so we designed a silent mutation.



Source

From E coli genome, obtained from iGEM team HZAU's lab. This plasmid is bought from Addgene Company.

References

Levskaya, A. et al (2005). Engineering Escherichia coli to see light. Nature, 438(7067), 442.

Olson E J, Hartsough L A, Landry B P, et al. Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals[J]. Nature methods, 2014, 11(4): 449-455. MLA