Difference between revisions of "Part:BBa K2136002"
Line 5: Line 5:
 
Lysostaphin is a zinc metalloproteinase (EC 3.4.24.75) produced by Staphylococcus simulans. This enzyme hydrolyses Glycine/Glycine bonds in the pentaglycine interpeptide that maintain the stability of peptidoglycans in staphylococcal cell wall [1]. Lysostaphin rapidly lyses actively growing and non-dividing cells including staphylococci in biofilms and due to its specificity, it could have high potential in the treatment of antibiotic-resistant staphylococcal infections [2]
 
Lysostaphin is a zinc metalloproteinase (EC 3.4.24.75) produced by Staphylococcus simulans. This enzyme hydrolyses Glycine/Glycine bonds in the pentaglycine interpeptide that maintain the stability of peptidoglycans in staphylococcal cell wall [1]. Lysostaphin rapidly lyses actively growing and non-dividing cells including staphylococci in biofilms and due to its specificity, it could have high potential in the treatment of antibiotic-resistant staphylococcal infections [2]
  
−
<!-- Add more about the biology of this part here
+
 
−
===Usage and Biology===
+
  
 
<!-- -->
 
<!-- -->
Line 12: Line 11:
 
<partinfo>BBa_K2136002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2136002 SequenceAndFeatures</partinfo>
  
−
PCR from pSB1C3 + Lysostaphin
 
  
 +
===Usage and Biology===
 +
 +
 +
<img src="https://static.igem.org/mediawiki/2016/9/93/T--USP_UNIFESP-Brazil--Lysosfromplasmid.png"
 +
<p class="fig-label"> <b>Figure 1.</b> PCR from pSB1C3 + Lysostaphin </p>
  
−
https://static.igem.org/mediawiki/2016/9/93/T--USP_UNIFESP-Brazil--Lysosfromplasmid.png">
 
−
<div class="foto-time" style="background-image: url('https://static.igem.org/mediawiki/2016/9/93/T--USP_UNIFESP-Brazil--Lysosfromplasmid.png')">
 
  
  

Revision as of 22:57, 17 October 2016


Lysostaphin

Lysostaphin is a zinc metalloproteinase (EC 3.4.24.75) produced by Staphylococcus simulans. This enzyme hydrolyses Glycine/Glycine bonds in the pentaglycine interpeptide that maintain the stability of peptidoglycans in staphylococcal cell wall [1]. Lysostaphin rapidly lyses actively growing and non-dividing cells including staphylococci in biofilms and due to its specificity, it could have high potential in the treatment of antibiotic-resistant staphylococcal infections [2]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 505
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

<img src="T--USP_UNIFESP-Brazil--Lysosfromplasmid.png"

Figure 1. PCR from pSB1C3 + Lysostaphin