Difference between revisions of "Part:BBa K1884009:Design"

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===Source===
 
===Source===
  
 +
PSAD promoter and terminator are gained from iGEM registry, AD-CRY2 and UAS are gained from Shenzhen Engineering Laboratory of Marine Algal Biotechnology.
  
 
   
 
   

Latest revision as of 02:13, 20 October 2016


PSAD Promoter+GalAD-CRY2+UAS+FNR(fumarate and nitrate reduction)+PSAD Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3123
    Illegal NotI site found at 3221
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1560
    Illegal BglII site found at 2019
    Illegal BamHI site found at 2498
    Illegal XhoI site found at 3052
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1444
    Illegal AgeI site found at 2173
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1796
    Illegal BsaI.rc site found at 1205
    Illegal SapI.rc site found at 1313
    Illegal SapI.rc site found at 3384


Design Notes

Source

PSAD promoter and terminator are gained from iGEM registry, AD-CRY2 and UAS are gained from Shenzhen Engineering Laboratory of Marine Algal Biotechnology.


References

[1]M. Winkler, A. Hemschemeier, J. Jacobs, S. Stripp, T. Happe Multiple ferredoxin isoforms in Chlamydomonas reinhardtii-their role under stress conditions and biotechnological implications Eur J Cell Biol, 89 (2010), pp. 998–1004

[2]I. Yacoby, S. Pochekailov, H. Toporik, M.L. Ghirardi, P.W. King, S.G. Zhang Photosynthetic electron partitioning between [FeFe]-hydrogenase and ferredoxin: NADP+-oxidoreductase (FNR) enzymes in vitro Proc Natl Acad Sci USA, 108 (2011), pp. 9396–9401

[3]M. Chen, L. Zhao, Y.L. Sun, S.X. Cui, L.F. Zhang, B. Yang, et al. Proteomic analysis of hydrogen photoproduction in sulfur-deprived Chlamydomonas cells J Proteome Res, 9 (2010), pp. 3854–3866