Difference between revisions of "Part:BBa K1974021"
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<h1>'''Introduction:'''</h1>
 
<h1>'''Introduction:'''</h1>
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[[File:|NCTU_H.png|800px|thumb|center|'''Figure 1.’’’T7 promoter+RBS+Hv1a+linker+His-tag+terminator ]]
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[[File:|NCTU_H.png|800px|thumb|center|'''Figure 1.’’’T7promoter+RBS+Hv1a+linker+snoedrop-lectin+linker+6xhistag ]]
  
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; By ligating the IPTG induced promoter T7 (BBa_ I712074), strong ribosome binding site (BBa_B0034), hv1a, linker, and the 6xHistag (BBa_ K1223006), we can express Hv1a, the toxin by IPTG induction  
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  
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By ligating the IPTG induced promoter T7 (BBa_ I712074), strong ribosome binding site (BBa_B0034), hv1a, linker, and the 6xHistag (BBa_ K1223006), we can express Hv1a, the toxin by IPTG induction  
 
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; This year we create a revolutionary system that integrates biological pesticides, automatic detector, sprinkler, and IoT. We made a database that contains most of the spider toxins and selected the target toxins by programming. Omega-hexatoxin-Hv1a is coded for the venom of a spider, <i>Hadronyche versuta</i>.
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  
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This year we create a revolutionary system that integrates biological pesticides, automatic detector, sprinkler, and IoT. We made a database that contains most of the spider toxins and selected the target toxins by programming. Omega-hexatoxin-Hv1a is coded for the venom of a spider, <i>Hadronyche versuta</i>.
  
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It is under the control of the strong T7 promoter. Snowdrop-lectin acts as a carrier that could transport the toxin to insect’s nervous system, hemolymph and can improve the oral activity. A 6xHistag is added for further protein purification.
  
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<p style="padding-top:20px;">
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<b>Mechanism of Hv1a:</b></p>
  
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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According to reference, snowdrop-lectin is resistant to high temperature and would not be degraded by digestive juice. The species-specificity is based on the toxin, and the snowdrop lectin is the role of the carrier.
  
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According to reference, Omega-hexatoxin-Hv1a has a structure called ICK(inhibitor cysteine knot).[1](之前PPT裡有) This kind of structure contains three disulfide bonds and beta-sheet. With this structure, Hv1a can resist the high temperature, acid-base solution and the digest juice of insect gut. Hv1a can bind on insect voltage-gated Calcium channels (CaV1) in the central nervous system, making it paralyze and die eventually.
  
  

Revision as of 17:17, 17 October 2016

T7Promoter+RBS+Hv1a+linker+snowdrop-lectin+linker+6X His-Tag


Introduction:

[[File:|NCTU_H.png|800px|thumb|center|Figure 1.’’’T7promoter+RBS+Hv1a+linker+snoedrop-lectin+linker+6xhistag ]]

       By ligating the IPTG induced promoter T7 (BBa_ I712074), strong ribosome binding site (BBa_B0034), hv1a, linker, and the 6xHistag (BBa_ K1223006), we can express Hv1a, the toxin by IPTG induction
       This year we create a revolutionary system that integrates biological pesticides, automatic detector, sprinkler, and IoT. We made a database that contains most of the spider toxins and selected the target toxins by programming. Omega-hexatoxin-Hv1a is coded for the venom of a spider, Hadronyche versuta.

       It is under the control of the strong T7 promoter. Snowdrop-lectin acts as a carrier that could transport the toxin to insect’s nervous system, hemolymph and can improve the oral activity. A 6xHistag is added for further protein purification.



Mechanism of Hv1a:

       According to reference, snowdrop-lectin is resistant to high temperature and would not be degraded by digestive juice. The species-specificity is based on the toxin, and the snowdrop lectin is the role of the carrier.

       According to reference, Omega-hexatoxin-Hv1a has a structure called ICK(inhibitor cysteine knot).[1](之前PPT裡有) This kind of structure contains three disulfide bonds and beta-sheet. With this structure, Hv1a can resist the high temperature, acid-base solution and the digest juice of insect gut. Hv1a can bind on insect voltage-gated Calcium channels (CaV1) in the central nervous system, making it paralyze and die eventually.









Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]