Difference between revisions of "Part:BBa K1993001"

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'''Figure 4. EF-1α-CCR7.'''
 
'''Figure 4. EF-1α-CCR7.'''
  
We introduced that plasmid into MSCs and tested the expression of CCR7 in MSCs on the protein level (Figure 5)
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We introduced that plasmid into MSCs and tested the expression of CCR7 in MSCs on mRNA and protein level (Figure 5, Figure 6)
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'''Figure 5. Relative mRNA Level of CCR7.'''
  
 
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'''Figure 5. Western Blot of CCR7.'''
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'''Figure 6. Western Blot of CCR7.'''
  
  
Then, we tested the chemotaxis of engineered MSCs by conducting Transwell assay against CCL19. To our excitement, our engineered MSCs had improved their homing ability with chemokine receptor CCR7(Figure 6).
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Then, we tested the chemotaxis of engineered MSCs by conducting Transwell assay against CCL19. To our excitement, our engineered MSCs had improved their homing ability with chemokine receptor CCR7(Figure 7).
  
 
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'''Figure 6. Tanswell Assay of CCR7.'''
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'''Figure 7. Tanswell Assay of CCR7.'''
  
 
==References==
 
==References==

Revision as of 17:16, 17 October 2016


CCR7

Chemokine receptors are receptors found on the surface of certain cells that interact with chemokines. They have a 7-transmembrane (7-TM) structure and couple to G-protein for signal transduction within a cell. [1] (Figure 1) Following interaction with their specific chemokine ligands, chemokine receptors trigger a flux intracellular calcium (Ca2+) ions, initiate chemotaxis and guide the cell to a desired location. (Figure 2)

Figure 1. typical structure of a chemokine receptor.


Figure 2. the mechanism of interaction between a chemokine and a chemokine receptor.


Under the circumstances of inflammation, various kinds of cytokines, including chemokines, are released by the lesions. Guided by the chemokines, cells expressing chemokine receptors move towards the lesions where they can function better.[2] What’s more, different diseases would release different pools of chemokines, which would recruit different effector cells. See our disease talbe-chemokine

Based on the chemotaxis theory, in order to enhance the homing ability of our marvelous mesenchymal stem cells (MSCs) due to lack of enough chemokine receptors on their cell surface, we, SYSU-MEDICINE, had constructed a series of chemokine receptors that corresponding to different inflammatory diseases as far as possible. Among which, CCL19 is a significant chemokine (CCR7 is its chemokine receptor) in Arthritis. See our disease talbe-chemokine

We acquired this gene from peripheral mononuclear blood cells (PMBCs) and purified it. (Figure 3) Then we constructed it under the control of EF-1α by Gateway technology. (Figure 4)

Figure 3. Purification of CCR7.

Figure 4. EF-1α-CCR7.

We introduced that plasmid into MSCs and tested the expression of CCR7 in MSCs on mRNA and protein level (Figure 5, Figure 6)

Figure 5. Relative mRNA Level of CCR7.

Figure 6. Western Blot of CCR7.


Then, we tested the chemotaxis of engineered MSCs by conducting Transwell assay against CCL19. To our excitement, our engineered MSCs had improved their homing ability with chemokine receptor CCR7(Figure 7).

Figure 7. Tanswell Assay of CCR7.

References

[1] Allen, Samantha J.; Crown, Susan E.; Handel, Tracy M. (2007-01-01). "Chemokine: receptor structure, interactions, and antagonism". Annual Review of Immunology. 25: 787–820.doi:10.1146/annurev.immunol.24.021605.090529. ISSN 0732-0582. PMID 17291188.

[2] Griffith J W, Sokol C L, Luster A D. Chemokines and chemokine receptors: positioning cells for host defense and immunity.[J]. Annual Review of Immunology, 2014, 32(1):659-702.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1111
    Illegal SapI site found at 1017