Difference between revisions of "Part:BBa K2117000"
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The translation elongation factor-1α (TEF1) promoter is a strong constitutive promoter.
 
The translation elongation factor-1α (TEF1) promoter is a strong constitutive promoter.
 
Native for the oleaginous yeast Yarrowia lipolytica.
 
Native for the oleaginous yeast Yarrowia lipolytica.
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This promoter is often used for isolation of enzyme genes by expression cloning. However, the promoter is usually not recommended for heterologous production due to early expression of heterologous genes can be detrimental for cell growth.
  
 
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===Usage and Biology===
 
===Usage and Biology===
  
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This promoter is often used for isolation of enzyme genes by expression cloning. However, the promoter is usually not recommended for heterologous production due to early expression of heterologous genes can be detrimental for cell growth.
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Revision as of 16:50, 17 October 2016


Constitutive TEF1 promoter native to Yarrowia lipolytica

The translation elongation factor-1α (TEF1) promoter is a strong constitutive promoter. Native for the oleaginous yeast Yarrowia lipolytica.

This promoter is often used for isolation of enzyme genes by expression cloning. However, the promoter is usually not recommended for heterologous production due to early expression of heterologous genes can be detrimental for cell growth.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2