Difference between revisions of "Part:BBa K1993033"

Latest revision as of 19:39, 17 October 2016

CXCR5-T2A-Luciferase

With the purpose of increasing homing efficiency of MSCs and illustrating their distribution, we had constructed a composite part that containing a chemokine receptor CXCR5 and a bioluminescence Luciferase. It’s also convenient for us to construct our plasmids through Gateway technology.

CXCR5 is responsible for homing to the lesions whose ligand is CXCL13, one of the most common chemokines in various inflammatory diseases, especially in delayed type hypersensitivity(DTH). (Details could be seen on BBa_K1993013).

Luciferase is a kind of oxidative enzymes that produce bioluminescence which enable us to observe cells in vivo. (Details could be seen on BBa_K1993015).

T2A is a self-cleaving 2A peptide (The average length is 18–22 amino acids) that could be applied in expression of more than one gene in cells. (Details could be seen on BBa_K1993019)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1398
Illegal BamHI site found at 1941
Illegal XhoI site found at 31
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 973
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 259
Illegal SapI site found at 1050

@@ Line 5: / Line 5: @@
 With the purpose of increasing homing efficiency of MSCs and illustrating their distribution, we had constructed a composite part that containing a chemokine receptor CXCR5 and a bioluminescence Luciferase. It’s also convenient for us to construct our plasmids through Gateway technology.
-CXCL13 is one of the most common chemokines in various inflammatory diseases, especially in delayed type hypersensitivity(DTH). CXCR5, its corresponding chemokine receptor, is responsible for homing to the lesions. (Details could be seen on [https://parts.igem.org/Part:BBa_K1993013 BBa_K1993013]).
+CXCR5 is responsible for homing to the lesions whose ligand is CXCL13, one of the most common chemokines in various inflammatory diseases, especially in delayed type hypersensitivity(DTH).  (Details could be seen on [https://parts.igem.org/Part:BBa_K1993013 BBa_K1993013]).
-Luciferase is a kind of oxidative enzymes that produce bioluminescence which enable us observing cells in vivo. (Details could be seen on [https://parts.igem.org/Part:BBa_K1993015 BBa_K1993015]).
+Luciferase is a kind of oxidative enzymes that produce bioluminescence which enable us to observe cells in vivo. (Details could be seen on [https://parts.igem.org/Part:BBa_K1993015 BBa_K1993015]).
 T2A is a self-cleaving 2A peptide (The average length is 18–22 amino acids) that could be applied in expression of more than one gene in cells. (Details could be seen on [https://parts.igem.org/Part:BBa_K1993019 BBa_K1993019])