Difference between revisions of "Part:BBa K1985012"
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<partinfo>BBa_K1985012 short</partinfo> | <partinfo>BBa_K1985012 short</partinfo> | ||
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+ | This part is an improved version of a previously designed BioBrick (Part:BBa_K1739002), which was designed by the Kent 2015 iGEM team. The promoter BBa_J23104 has been removed. The part contains two segments in one biobrick: CsgA signal sequence and Sup35NM. | ||
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<partinfo>BBa_K1985012 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1985012 SequenceAndFeatures</partinfo> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
For more information on biology and usage see part [https://parts.igem.org/Part:BBa_K1739002 BBa_K1739002] | For more information on biology and usage see part [https://parts.igem.org/Part:BBa_K1739002 BBa_K1739002] | ||
+ | ===Validation=== | ||
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+ | The part was first validated with a diagnostic restriction digest using EcoRI and PstI and agarose gel electrophoresis. The expected band sizes from the digest were: xxxx for the plasmid backbone and xxx for the insert. A 1kB plus DNA marker was used to verify the sizes of the bands and it was confirmed that the correct plasmid had been produced. | ||
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+ | [[File:filename.jpeg||400px|thumb|centre|Figure 1. Agarose gel of the restriction digest of BBa_K1985012 in pSB1C3, with EcoRI and PstI.]] | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display |
Revision as of 15:20, 17 October 2016
[CsgA-SS-Sup35NM]
This part is an improved version of a previously designed BioBrick (Part:BBa_K1739002), which was designed by the Kent 2015 iGEM team. The promoter BBa_J23104 has been removed. The part contains two segments in one biobrick: CsgA signal sequence and Sup35NM.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 671
Usage and Biology
For more information on biology and usage see part BBa_K1739002
Validation
The part was first validated with a diagnostic restriction digest using EcoRI and PstI and agarose gel electrophoresis. The expected band sizes from the digest were: xxxx for the plasmid backbone and xxx for the insert. A 1kB plus DNA marker was used to verify the sizes of the bands and it was confirmed that the correct plasmid had been produced.