Difference between revisions of "Part:BBa K1899005:Experience"

Revision as of 14:57, 17 October 2016

Applications of BBa_K1899005

Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240 and pSB3K3-PhlFp- BBa_E0240

The fold change between pSB3K3-PhlFp - BBa_E0240 and negative control (pSB3K3-BBa_E0240) and that of pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240 is around 13.2 times and 7.01 times respectively.

This is due to the toxicity of <bbcode>BBa_C0040 <bbcode>(TetR). The toxicity reduces the growth rate of the E. coli containing this plasmid. The strong promoter BBa_J23101 makes this effect more significant when doing the characterisation. Though the data are collected with all OD within the mid-log range, there is still a distance between them, making a significant difference in the RFU. It is ungrounded to say TetR interferes the functionality of PhlFp at this stage.

User Reviews

UNIQ471ecd16ece66da7-partinfo-00000000-QINU UNIQ471ecd16ece66da7-partinfo-00000001-QINU

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 __NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
-how you used this part and how it worked out.
 ===Applications of BBa_K1899005===
 https://static.igem.org/mediawiki/parts/thumb/2/25/Comparison_of_the_Strength_of_tetR_and_PhlFp.jpeg/748px-Comparison_of_the_Strength_of_tetR_and_PhlFp.jpeg
-<b>Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240 and  pSB3K3-<i>PhlFp</i>(<bbpart>_BBa_K1899004<bbpart>_  BBa_E0240</b>
+<b>Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240 and  pSB3K3-<i>PhlFp</i>- BBa_E0240</b>
 The fold change between<b> pSB3K3-PhlFp - BBa_E0240</b> and negative control<b> (pSB3K3-BBa_E0240)</b> and that of <b>pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240</b> is around 13.2 times and 7.01 times respectively.