Difference between revisions of "Part:BBa K1937006"
(→‎Background:)
 
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==Background:==
 
==Background:==
−
pSBBS0K-mini <i>B. subtilis</i> plasmid containing a pNagP-RFP construction. The pNagP promoter of the NagP gene can induce expression in presence of N-acetylglucosamine 6-phosphate. <br>
+
pSBBs0K-mini <i>B. subtilis</i> plasmid containing a pNagP-RFP construction. The pNagP promoter of the NagP gene can induce expression in presence of N-acetylglucosamine 6-phosphate. <br>
 
[[File:BBa_K1937006-completemap.png]]<br>
 
[[File:BBa_K1937006-completemap.png]]<br>
 
This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)
 
This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)

Latest revision as of 19:34, 17 October 2016


pNagP-RFP in pSBBs0K-mini plasmid

(Chassis B. subtilis, carrier plasmid pSBBS0K-mini) Length: 6843 bp

Background:

pSBBs0K-mini B. subtilis plasmid containing a pNagP-RFP construction. The pNagP promoter of the NagP gene can induce expression in presence of N-acetylglucosamine 6-phosphate.
BBa K1937006-completemap.png
This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1278
    Illegal suffix found in sequence at 1306
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1278
    Illegal NheI site found at 222
    Illegal NheI site found at 1300
    Illegal SpeI site found at 1307
    Illegal PstI site found at 1321
    Illegal NotI site found at 7
    Illegal NotI site found at 1284
    Illegal NotI site found at 1314
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1278
    Illegal BglII site found at 19
    Illegal BglII site found at 1020
    Illegal BglII site found at 5835
    Illegal BamHI site found at 1351
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1307
    Illegal EcoRI site found at 1278
    Illegal XbaI site found at 1293
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 1278
    Illegal XbaI site found at 16
    Illegal XbaI site found at 1293
    Illegal SpeI site found at 1307
    Illegal PstI site found at 1321
    Illegal NgoMIV site found at 2898
    Illegal AgeI site found at 782
    Illegal AgeI site found at 894
    Illegal AgeI site found at 5446
    Illegal AgeI site found at 6408
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1076
    Illegal BsaI.rc site found at 2722
    Illegal BsaI.rc site found at 4161
    Illegal BsaI.rc site found at 6677
    Illegal SapI site found at 1639
    Illegal SapI.rc site found at 5659