Difference between revisions of "Part:BBa K1899005:Experience"
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===Applications of BBa_K1899005=== | ===Applications of BBa_K1899005=== | ||
https://static.igem.org/mediawiki/parts/thumb/2/25/Comparison_of_the_Strength_of_tetR_and_PhlFp.jpeg/748px-Comparison_of_the_Strength_of_tetR_and_PhlFp.jpeg | https://static.igem.org/mediawiki/parts/thumb/2/25/Comparison_of_the_Strength_of_tetR_and_PhlFp.jpeg/748px-Comparison_of_the_Strength_of_tetR_and_PhlFp.jpeg | ||
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| − | + | <b>Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240 and pSB3K3-<i>PhlFp</i>(<bbpart>_BBa_K1899004<bbpart>_ BBa_E0240</b> | |
| − | + | The fold change between<b> pSB3K3-PhlFp - BBa_E0240</b> and negative control<b> (pSB3K3-BBa_E0240)</b> and that of <b>pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240</b> is around 13.2 times and 7.01 times respectively. | |
| + | This is due to the toxicity of <bbcode>BBa_C0040 <bbcode>(TetR). The toxicity reduces the growth rate of the<i> E. coli</i> containing this plasmid. The strong promoter BBa_J23101 makes this effect more significant when doing the characterisation. Though the data are collected with all OD within the mid-log range, there is still a distance between them, making a significant difference in the RFU. It is ungrounded to say TetR interferes the functionality of <i> PhlFp</i> at this stage. | ||
===User Reviews=== | ===User Reviews=== | ||
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Revision as of 14:56, 17 October 2016
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Applications of BBa_K1899005
Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240 and pSB3K3-PhlFp(_BBa_K1899004