Difference between revisions of "Part:BBa K2065000:Experience"
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==iGEM TU Eindhoven 2016== | ==iGEM TU Eindhoven 2016== | ||
| − | iGEM TU Eindhoven used this part in combination with CT52-LargeBiT (BBa_K2065007). These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. Thus this system was used as a read out method for T14-3-3 heterodimers. | + | iGEM TU Eindhoven used this part in combination with CT52-LargeBiT (BBa_K2065007). These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. Thus this system was used as a read out method for T14-3-3 heterodimers. This system is schemtically visualised below. For more detailed information about the application of CT52-SmallBiT visit our wiki. |
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| + | [[File:T--TU-Eindhoven--NanolUcreadoutbb.png]] | ||
| + | <br> | ||
| + | ''Measurement system for T14-3-3 heterodimers using the NanoBiT system linked to CT52'' | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 23:40, 18 October 2016
Applications of BBa_K2065000
iGEM TU Eindhoven 2016
iGEM TU Eindhoven used this part in combination with CT52-LargeBiT (BBa_K2065007). These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. Thus this system was used as a read out method for T14-3-3 heterodimers. This system is schemtically visualised below. For more detailed information about the application of CT52-SmallBiT visit our wiki.
Measurement system for T14-3-3 heterodimers using the NanoBiT system linked to CT52
User Reviews
UNIQ397f02517e3457b0-partinfo-00000000-QINU UNIQ397f02517e3457b0-partinfo-00000001-QINU