Difference between revisions of "Part:BBa K2065000:Experience"
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===Applications of BBa_K2065000=== | ===Applications of BBa_K2065000=== | ||
| − | ==iGEM TU Eindhoven== | + | ==iGEM TU Eindhoven 2016== |
iGEM TU Eindhoven used this part in combination with CT52-LargeBiT (BBa_K2065007). These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. Thus this system was used as a read out method for T14-3-3 heterodimers. | iGEM TU Eindhoven used this part in combination with CT52-LargeBiT (BBa_K2065007). These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. Thus this system was used as a read out method for T14-3-3 heterodimers. | ||
Revision as of 11:55, 17 October 2016
Applications of BBa_K2065000
iGEM TU Eindhoven 2016
iGEM TU Eindhoven used this part in combination with CT52-LargeBiT (BBa_K2065007). These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. Thus this system was used as a read out method for T14-3-3 heterodimers.
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