Difference between revisions of "Help:Submission Kit"
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Revision as of 15:40, 1 June 2017
- Registry Help Pages:
- TOC
- At-a-Glance
- FAQ
Requirements
Here's how part samples are sent to the Registry
Why pSB1C3?
Why do samples need to be shipped in pSB1C3?
Detailed Instructions
Instructions on how to fill out the Registry's submission form.
Submission Prep
Registry groups must follow iGEM's submission prep guidelines.
Contents
Submission Format
For iGEM 2016, we are standardizing the part submission process.
As in previous years, parts must be BioBrick compatible and in pSB1C3.
Additionally, all part submissions must be:
- miniprepped plasmid DNA (over 250ng) dried down. 10ul of 25ng/ul for a total of 250ng works well.
- in 96 well plate format (label your plate with your team name and submission number)
- plate sealed with adhesive foil
- the adhesive foil should make full contact with the wells of the plate (see picture)
- sealed plate covered with a plastic lid
- shipped with a tracking number
- shipped and labelled as DNA, not hidden or disguised
- DNA (non-hazardous, non-regulated, non-infectious, for research purposes only)
Submission Kit Contents
NOTE: The Submission Kit is new to iGEM! If you have any questions about using it, please don't hesitate to contact us at hq (at) igem (dot) org
- (3) 96-well skirted PCR plates
- (3) universal plate lids
- (5) adhesive foils
- bubble wrap
- a box with the iGEM HQ address, for shipping your submission
Sample Layout
Make sure to add your samples in the correct layout:
- Your first sample (s1) should be dispensed into well 1A of your submission plate.
- Your second sample (s2) should be dispensed into well 1B of the same submission plate.
Use one plate per a submission order. Example: If you are submitting 104 parts, create one submission order for 96 parts and another submission order for 8 parts. Label the plates with the correct submission order.
Submission DNA Preparation
- 250ng minimum of purified plasmid DNA (your BioBrick compatible part in pSB1C3)
- 10ul of a 25ng/ul miniprep will work well
- DNA must be dried down before shipping
We recommend drying down the plate in your lab's laminar flow hood (you can also leave your plate on the lab bench to air-dry, covered with the plastic lid).
- Sterilize the hood surface with 70% Ethanol
- Cover the 96 well plate with the provided plate lid (not with the adhesive foil)
- Place the plate in the hood.
- With sufficient airflow, drying down 10ul of 25ng/ul (250ng total) of purified plasmid DNA in the plate should take 4-6 hours (or overnight).
- Once all wells have dried, cover the plate wells with the provided adhesive foil.
- The adhesive foil should make full contact with the wells of the plate (see picture). When properly sealed you should be able to see the impression of every well.
- Cover the sealed plate with a plastic lid
- Label your plate
Plate Labelling
Make sure to label each plate with your group (team/lab) name and the appropriate submission number. Use a lab marker and write your team name and submission number on the side of the 96w plate.
Use one plate per a submission order. Example: If you are submitting 104 parts, create one submission order for 96 parts and another submission order for 8 parts. Label the plates with the correct submission order.
Packaging your submission for shipping
Place your submission in an appropriately sized shipping container, such as a cardboard box, along with the printout of the submission form. If you use cushioning material inside the container, limit cushioning material to foam peanuts, bubble wrap, packaging paper, or similar.
Note: Part submissions packaged in materials other than the above will be rejected and these part submissions will not be taken into consideration for medals or awards. It is important that you not hide your submissions in an effort to conceal them from customs, because they will be seized.
IMPORTANT: iGEM 2016 Teams in China
For 2016, iGEM HQ and the Registry are collaborating with Genscript to help iGEM Teams in China submit their part samples. In the past, some teams from China have had issues shipping to the United States. With this offer, iGEM Teams in China will ship their part samples to Genscript, and Genscript will forward these shipments to iGEM HQ.
Genscript Shipping Details
- Ship to address: 江苏省南京市江宁区雍熙路28号 金斯瑞生物科技
- Receiver: 刘吉平
- Tel: 400-025-8686转分机号5841
- Email: jiping.liu@genscript.com
All iGEM Teams in China must still follow the normal directions for part submission:
- miniprepped plasmid DNA (over 250ng) dried down. 10ul of 25ng/ul for a total of 250ng works well.
- in 96 well plate format (label your plate with your team name and submission number)
- plate sealed with adhesive foil
- the adhesive foil should make full contact with the wells of the plate (see picture)
- sealed plate covered with a plastic lid
- shipped with a tracking number
- shipped and labelled as DNA, not hidden or disguised
- DNA (non-hazardous, non-regulated, non-infectious, for research purposes only)