Difference between revisions of "Part:BBa K2066057:Design"
Line 14: Line 14:
 
This part was created from existing parts using gibson assembly.  
 
This part was created from existing parts using gibson assembly.  
  
−
The UNS2, UNS3, and UNS6 sequences are taken from Torella et al. 2013 (“Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly”). RiboJ sequence is from Lou et. al 2012 ("Ribozyme-based insulator parts buffer synthetic circuits from genetic context").
+
The UNS2 and UNS3 sequences are taken from Torella et al. 2013 (“Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly”). RiboJ sequence is from Lou et. al 2012 ("Ribozyme-based insulator parts buffer synthetic circuits from genetic context").
  
 
===References===
 
===References===

Revision as of 23:09, 16 October 2016


J23100+RiboJ+sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 181


Design Notes

This part is K2066014 with a different promoter. Characterization data from this part should be compared with K2066058 to determine effect of RiboJ.


Source

This part was created from existing parts using gibson assembly.

The UNS2 and UNS3 sequences are taken from Torella et al. 2013 (“Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly”). RiboJ sequence is from Lou et. al 2012 ("Ribozyme-based insulator parts buffer synthetic circuits from genetic context").

References