Difference between revisions of "Part:BBa K2086001"

Revision as of 21:21, 16 October 2016

Serine Repeat Antigen (SerA)

The E. coli serA gene encodes the D-3-phosphoglycerate dehydrogenase, which catalyzes the first committed step in the biosynthesis of serine. Serine is an essential amino acid for E. coli growth in minimal medium. Deletion of the serA gene leads to a serine auxotroph, which can be rescued either by the expression of a protein with the same catalytic activity as SerA or by the addition of serine in the growth media.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 293
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Usage and Biology

Serine is an amino acid produced in E. coli K12 through the following metabolic pathway.

Figure 1: The serine biosynthesis pathway.

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	Serine is an amino acid produced in E. coli K12 through the following metabolic pathway.		Serine is an amino acid produced in E. coli K12 through the following metabolic pathway.
−	[[Image:Bielefeld2012_ECOL3LFermentation.jpg\|450px\|thumb\|~~left~~\|'''Figure 1:''' The serine biosynthesis pathway' KRXwith <partinfo>BBa_K863005</partinfo> (ECOL) in an Infors Labfors Bioreactor, scale: 3 L, [http://2012.igem.org/Team:Bielefeld-Germany/Protocols/Materials#Autoinduction_medium autoinduction medium] + 60 µg/mL chloramphenicol, 37 °C, pH 7, agitation on cascade to hold pO<sub>2</sub> at 50 %, OD<sub>600</sub> measured every 30 minutes.]]	+	[[Image:Bielefeld2012_ECOL3LFermentation.jpg\|450px\|thumb\|right\|'''Figure 1:''' The serine biosynthesis pathway.]]