Difference between revisions of "Part:BBa K1956014"
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== Experimentation ==
 
== Experimentation ==
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This BioBrick was used in <i>E. coli</i> DH5alpha to estimate intrinsic noise. Expression data was obtained using flow cytometry. OD600 values for specific growth curve were obtained using Spectrophotometer over an interval of an hour for 12 hours. Given specific growth rates are in it's logarithmic values.   
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<p>This BioBrick was used in <i>E. coli</i> DH5alpha to estimate intrinsic noise. Expression data was obtained using flow cytometry. OD600 values for specific growth curve were obtained using Spectrophotometer over an interval of an hour for 12 hours. Given specific growth rates are in it's logarithmic values.  </p>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 20:37, 16 October 2016


Noise in Device measurement

Noise in Device measurement 2

Usage and Biology

IIT Madras 2016's Characterization

Specific growth rates (ln) for Devices
Relative strength of Devices

Experimentation

This BioBrick was used in E. coli DH5alpha to estimate intrinsic noise. Expression data was obtained using flow cytometry. OD600 values for specific growth curve were obtained using Spectrophotometer over an interval of an hour for 12 hours. Given specific growth rates are in it's logarithmic values.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1728
    Illegal AgeI site found at 1840
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 726