Difference between revisions of "Part:BBa K2170001:Design"
Line 9: | Line 9: | ||
'''Related BioBrick:''' | '''Related BioBrick:''' | ||
− | + | Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170002 BBa_K2170002: Secretory eukaryotic biotin binding receptor with single chain avidin] | |
− | + | Related BioBricks:[https://parts.igem.org/wiki/index.php?title=BBa_K2170003 BBa_K2170003: Biotinylated receptor with biotin acceptor peptide combined with a secretory BirA] | |
'''Cloning details:'''<br> | '''Cloning details:'''<br> | ||
− | + | Designed in RFC25 | |
− | + | Mutation C889G to delete XbaI restriction site | |
− | + | Truncation upstream/downstream compared to template, ?explanation? | |
'''Quality control measures:'''<br> | '''Quality control measures:'''<br> | ||
− | + | Test digestion using EcorRI & PstI | |
− | + | Sequencing using VF2 | |
− | + | Part was partly sequenced | |
'''Backbone:'''<br> | '''Backbone:'''<br> | ||
− | + | Backbone name: pSB1C3 | |
− | + | Resistance: Cp | |
− | <!--*Copynumber: | + | <!--*Copynumber: high |
'''Protein coding:'''<br> | '''Protein coding:'''<br> | ||
− | + | Protein: Secretory eukaryotic biotin binding receptor with single chain avidin [Nucleotide 633 to 3314] | |
− | + | ||
− | + | ||
− | + | ||
'''Enzymatic activity:''' | '''Enzymatic activity:''' | ||
− | + | none | |
'''Cytotoxicity:'''<br> | '''Cytotoxicity:'''<br> | ||
− | + | not known | |
'''Safety notes:'''<br> | '''Safety notes:'''<br> | ||
− | + | Known and anticipated sefety issues: none | |
− | <!--Known and anticipated security issues: none | + | <!--Known and anticipated security issues: none |
'''Intellectual property:''' | '''Intellectual property:''' | ||
Line 57: | Line 56: | ||
<!--*Preexisting BioBrick ?Bba_number?--> | <!--*Preexisting BioBrick ?Bba_number?--> | ||
<!--*cDNA Clone: ?clone_name?, ?company_name?--> | <!--*cDNA Clone: ?clone_name?, ?company_name?--> | ||
− | + | Parts synthesized by IDT | |
<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>--> | <!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>--> | ||
Line 74: | Line 73: | ||
'''Literature references:'''<br> | '''Literature references:'''<br> | ||
− | <!--*[http://www. | + | <!--*[http://www.nature.com/nmeth/journal/v2/n2/full/nmeth735.html >Chen, I., Howarth, M., Lin, W., & Ting, A. Y. (2005). Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase. Nature methods, 2(2), 99-104.]--> |
'''Database references:'''<br> | '''Database references:'''<br> |
Revision as of 12:55, 19 October 2016
Secretory eukaryotic biotin binding receptor with enhanced monomeric avidin
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2432
Design Notes
Related BioBrick: Other versions:BBa_K2170002: Secretory eukaryotic biotin binding receptor with single chain avidin Related BioBricks:BBa_K2170003: Biotinylated receptor with biotin acceptor peptide combined with a secretory BirA
Cloning details:
Designed in RFC25
Mutation C889G to delete XbaI restriction site
Truncation upstream/downstream compared to template, ?explanation?
Quality control measures:
Test digestion using EcorRI & PstI
Sequencing using VF2
Part was partly sequenced
Backbone:
Backbone name: pSB1C3
Resistance: Cp
Corresponding part author/authors:
Source
Source:
Parts synthesized by IDT
Organism:
Genesequence derived from Escherichia coli
References
Literature references:
Database references: