Difference between revisions of "Part:BBa K2066040:Design"

(→‎Source)
(→‎Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
to be completed
 
  
  
 +
UNS standard chosen for ease of cloning with standard primers.
  
 
===Source===
 
===Source===

Revision as of 17:48, 16 October 2016


J23100 + RiboJ Promoter Characterization Part with 2x Broccoli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

UNS standard chosen for ease of cloning with standard primers.

Source

The UNS2, UNS3, and UNS6 sequences are taken from Torella et al. 2013 (“Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly”). The spacer sB is taken from Pothoulakis et al. 2013 (“The Spinach RNA Aptamer as a Characterization Tool for Synthetic Biology”). The broccoli sequence is from https://www.addgene.org/66843/. The mCherry sequence is taken from WM16_029 with the start codon and extra stop codon added.

References