Difference between revisions of "Part:BBa I712009"
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=Additional characterization of BBaI712009 by [http://2016.igem.org/Team:LMU-TUM_Munich Munich 2016]:= | =Additional characterization of BBaI712009 by [http://2016.igem.org/Team:LMU-TUM_Munich Munich 2016]:= | ||
| − | [[File:Muc16_CD4_signal_peptide_001.png|thumb|center|920px| Sequencing results of BioBrick[https://parts.igem.org/wiki/index.php?title=Part: | + | [[File:Muc16_CD4_signal_peptide_001.png|thumb|center|920px| Sequencing results of BioBrick[https://parts.igem.org/wiki/index.php?title=Part:BBa_I712009] & consequential ligation problems]] |
This part was submitted before the RFC[25] standard was established. Thus this part carries a modified RFC[10] pre- and suffix that changes the reading frame of the suffix in a way that, if it is ligated to a second part, it does not generate a stop codon. Although this approach makes it possible to assemble fusion proteins, the ligation to a second part, which does not carry the modified RFC[10], leads to a frameshift in the second part in a way that it is not functional anymore. | This part was submitted before the RFC[25] standard was established. Thus this part carries a modified RFC[10] pre- and suffix that changes the reading frame of the suffix in a way that, if it is ligated to a second part, it does not generate a stop codon. Although this approach makes it possible to assemble fusion proteins, the ligation to a second part, which does not carry the modified RFC[10], leads to a frameshift in the second part in a way that it is not functional anymore. | ||
Revision as of 17:15, 16 October 2016
CD4 signal peptide; localizes to plasma membrane
Additional characterization of BBaI712009 by [http://2016.igem.org/Team:LMU-TUM_Munich Munich 2016]:
This part was submitted before the RFC[25] standard was established. Thus this part carries a modified RFC[10] pre- and suffix that changes the reading frame of the suffix in a way that, if it is ligated to a second part, it does not generate a stop codon. Although this approach makes it possible to assemble fusion proteins, the ligation to a second part, which does not carry the modified RFC[10], leads to a frameshift in the second part in a way that it is not functional anymore.
Because signal peptides are such important BioBricks we designed new signal peptide parts such as BBa_K2170215 and BBa_K2170214 to help other iGEM teams assembling fusion proteins together.
Coding region for first 25 amino acids (including start codon) of CD4 receptor which is signal peptide for plasma membrane localization.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]