Difference between revisions of "Part:BBa K2066037"
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<partinfo>BBa_K2066037 short</partinfo>
 
<partinfo>BBa_K2066037 short</partinfo>
  
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This is the pACT-Tet sequence from Amit et al. 2011 “Building Synthetic Enhancers from the Ground Up” paper flanked by UNS 2/3. This is meant for use in Tet based synthetic enhancer systems.
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This is the pACT-Tet sequence from Amit et al. 2011 “Building Synthetic Enhancers from the Ground Up” paper on the UNS standard. This part consists of the endogenous nitrgoen regulatory promoter, the NRII mutant  BBa_K20660112 and tetR.  This is meant for use in Tet based synthetic enhancer systems.  
  
 
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Revision as of 03:09, 29 October 2016


pACT-Tet on UNS Standard

This is the pACT-Tet sequence from Amit et al. 2011 “Building Synthetic Enhancers from the Ground Up” paper on the UNS standard. This part consists of the endogenous nitrgoen regulatory promoter, the NRII mutant BBa_K20660112 and tetR. This is meant for use in Tet based synthetic enhancer systems.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 969
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2343
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]