Difference between revisions of "User:SBurke"

Revision as of 16:11, 20 August 2007

Assembly was the same as the one on 6/7/2007 except it used the old construction plasmid (prepped by ML on 3.13)
Tested transformation of 1ul of pSB1AK3 (LIGATED) in 50ul cells, plated all out on AK plates
Transform 1ul into 50ul cells (Top10-0008), do transformation on thermal module. Plate all 251ul on correct antibiotic plates.
Began counting colonies at 12:45PM

Construct color	Assembly construct	Number of colonies 1ul ~22hrs
RFP	A. 1AC3.J04500.I13501	167/184
GFP	B. 1AC3.R0010.I13504	80/91
RFP	C. 1AT3.J04500.I13501	41/61
GFP	D. 1AT3.R0010.I13504	24/40
RFP	E. 1AK3.J04500.I13501	18/811
GFP	F. 1AK3.R0010.I13504	0
	X-S control	69 (250ul plated)
	pUC control (in lig prod as usual)	0 (250ul plated)
	RFP J04450	734/734 (plated 50ul; 250ul plating yields 3670)
	pSB1AK3 (ligated)	41 (250ul plated)

Revision as of 16:06, 23 July 2007 (view source) SBurke (Talk \| contribs) ← Older edit		Revision as of 16:11, 20 August 2007 (view source) SBurke (Talk \| contribs) Newer edit →
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		+	[[Problems with PCR using VR/VF2]]