Difference between revisions of "Part:BBa K1884011"

Revision as of 14:12, 16 October 2016

RBCS2 Promoter+Paromomycin+RBCS2 Terminator

We provided Paromomycin resistance under the RBCS2 promoter(BBa_K1884010)

Usage and Biology

RBCS2 promoter

Paromoycin is a protein synthesis inhibitor in nonresistant cells by binding to 16S ribosomal RNA. This broad-spectrum antibiotic soluble in water, is very similar in action to neomycin. Antimicrobial activity of paromomycin against Escherichia coli and Staphylococcus aureus has been shown.

RBCS2 terminator is Chlamydomonas reinhardtii RuBisCO small subunit 2 terminator which obtained from the pOpt_mVenus_Hyg expression plasmid provided by Chlamydomonas Resource Center.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 14:11, 16 October 2016 (view source) Nllenwalker (Talk \| contribs) ← Older edit		Revision as of 14:12, 16 October 2016 (view source) Nllenwalker (Talk \| contribs) Newer edit →
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	Paromoycin is a protein synthesis inhibitor in nonresistant cells by binding to 16S ribosomal RNA. This broad-spectrum antibiotic soluble in water, is very similar in action to neomycin. Antimicrobial activity of paromomycin against Escherichia coli and Staphylococcus aureus has been shown.		Paromoycin is a protein synthesis inhibitor in nonresistant cells by binding to 16S ribosomal RNA. This broad-spectrum antibiotic soluble in water, is very similar in action to neomycin. Antimicrobial activity of paromomycin against Escherichia coli and Staphylococcus aureus has been shown.

−	RBCS2 terminator	+	RBCS2 terminator is Chlamydomonas reinhardtii RuBisCO small subunit 2 terminator which obtained from the pOpt_mVenus_Hyg expression plasmid provided by Chlamydomonas Resource Center.