Difference between revisions of "Part:BBa K1884012"

Revision as of 20:30, 16 October 2016

RBCS2 Promoter+Hygromycin B+RBCS2 Terminator

We provided Hygromycin B resistance under the RBCS2 promoter(BBa_K1884010)

Biology

Usage

For testing the efficiency of Hygromycin B, we formulated six gradient concentrations of Hygromycin B solution and add 200ul each into six different beakerflask which contain 200ml LB solid medium, then we put the competent cell into six different LB plate. there is a photograph to show several monoclonal colony growing on a LB plate which has the best selected abilty of Hygromycin B.(Fig 1) Conbined with another resistant device(BBa_K1884011), we are able to screen the green algae which has paromoycin resistance and Hygromycin B resistance. we added 200ul paromoycin solution and hygromycin B solution into 200ml TAP solid medium, then we put the green algae without cytoderm into TAP plate. there is a photograph to show several colony growing on a TAP plate which has the best selected efficiency of paromoycin and Hygromycin B.(Fig 2)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 650
Illegal NgoMIV site found at 832
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1105

@@ Line 5: / Line 5: @@
 We provided Hygromycin B resistance under the RBCS2 promoter(BBa_K1884010)
-===Usage and Biology===
+===Biology===
+===Usage===
+For testing the efficiency of Hygromycin B, we formulated six gradient concentrations of Hygromycin B solution and add 200ul each into six different beakerflask which contain 200ml LB solid medium, then we put the competent cell into six different LB plate. there is a photograph to show several monoclonal colony growing on a LB plate which has the best selected abilty of Hygromycin B.(Fig 1)
+Conbined with another resistant device(BBa_K1884011), we are able to screen the green algae which has paromoycin resistance and Hygromycin B resistance. we added 200ul paromoycin solution and hygromycin B solution into 200ml TAP solid medium, then we put the green algae without cytoderm into TAP plate. there is a photograph to show several colony growing on a TAP plate which has the best selected efficiency of paromoycin and Hygromycin B.(Fig 2)