Difference between revisions of "Part:BBa K2040121"
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<partinfo>BBa_K2040121 short</partinfo>
 
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KillerRed([https://parts.igem.org/Part:BBa_K1184000 BBa_K1184000]) is a red fluorescent protein that produces reactive oxygen species (ROS) in the presence of yellow-orange light (540-585 nm). KillerRed is engineered from anm2CP to be phototoxic.  Expression of KillerRed and irradiation with light may act a kill-switch for biosafety applications. More details about KillerRed see [http://2013.igem.org/Team:Carnegie_Mellon/KillerRed 2013 Carnegie_Mellon].
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A KillerRed expression cassette was created with a fungal promoter PgpdA and a fungal terminator TtrpC.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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KillerRed,always used for precise inactivation of selected proteins in chromophore-assisted light inactivation (CALI) technique and for the light-induced cell killing, is rarely used to express in fungal chassis.
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NYMU 2016 construct this new device, for fungal expression, to test wheather KillerRed protein can be used in entomopathogenic fungi ''Metarhizium anisopliae'', our chassis organism.
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We demonstrated the functionality of this part via transforming this expression cassette into ''M.anisopliae''. The transformants were under a fluorescence microscope(Magnification: 400×):
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*<b>Wildtype:</b>
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[[Image:WT FL.png|center|800px]]
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*<b>KillerRed transformant:</b>
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[[Image:KR FL.png|center|850px]]
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KillerRed proteins were successfully expressed in ''M.anisopliae''.
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Revision as of 20:53, 18 October 2016


PgpdA + KillerRed + TtrpC

A KillerRed expression cassette was created with a fungal promoter PgpdA and a fungal terminator TtrpC.


Usage and Biology

KillerRed,always used for precise inactivation of selected proteins in chromophore-assisted light inactivation (CALI) technique and for the light-induced cell killing, is rarely used to express in fungal chassis.

NYMU 2016 construct this new device, for fungal expression, to test wheather KillerRed protein can be used in entomopathogenic fungi Metarhizium anisopliae, our chassis organism.

We demonstrated the functionality of this part via transforming this expression cassette into M.anisopliae. The transformants were under a fluorescence microscope(Magnification: 400×):


  • Wildtype:
WT FL.png
  • KillerRed transformant:
KR FL.png


KillerRed proteins were successfully expressed in M.anisopliae.









Sequence and Features BBa_K2040121 SequenceAndFeatures