Difference between revisions of "Part:BBa K1933002"

Revision as of 15:17, 17 October 2016

anti-Norovirus GII.4 scFv

This part codes for anti-Norovirus GII.4 scFv (single-chain variable fragment) which derives from human 12A2 antibody. This scFv is suggested to prevent binding of human norovirus to Hist-Blood Group Antigens[1].
This coding sequence was fused to INPNC-His (BBa_K1933001) or BclA-His (BBa_K1933000) in our project.
For more information, please visit [http://2016.igem.org/Team:Kyoto our wiki]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 387
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Usage and Biology

We used this part for construction of BBa_K1933200 and BBa_K1933201.

Characterization

Please see BBa_K1933200 and BBa_K1933201 for full characterization.

Reference

[1]Higo‐Moriguchi, Kyoko, et al. "Isolation of cross‐reactive human monoclonal antibodies that prevent binding of human noroviruses to histo‐blood group antigens." Journal of medical virology, 86.4, (2014): 558-567.

@@ Line 14: / Line 14: @@
 ==Usage and Biology==
+<br>We used this part for construction of [https://parts.igem.org/Part:BBa_K1933200 BBa_K1933200] and [https://parts.igem.org/Part:BBa_K1933201 BBa_K1933201].
 ==Characterization==