Difference between revisions of "Part:BBa K1871010"
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<partinfo>BBa_K1871010 short</partinfo> | <partinfo>BBa_K1871010 short</partinfo> | ||
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| − | + | This is an AMY gene obtained from a Bacillus strain of bacteria. It produces an enzyme that hydrolyzes starch and degrades it into monosaccarides. | |
| − | This is an | + | |
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====Alpha Amylase==== | ====Alpha Amylase==== | ||
| − | + | α-Amylase is a protein enzyme that hydrolyses alpha bonds of long alpha-linked polysaccharides, such as glycogen. | |
====ADH1 Terminator==== | ====ADH1 Terminator==== | ||
| − | This part is the terminator region from yeast alcohol dehydrogenase | + | This part is the terminator region from yeast alcohol dehydrogenase gene. |
Latest revision as of 03:15, 16 October 2016
Coding Sequence AMY1
This is an AMY gene obtained from a Bacillus strain of bacteria. It produces an enzyme that hydrolyzes starch and degrades it into monosaccarides.
Native Alpha Amylase Secretion Sequence
This is the Bacillus amyloliquefaciens alpha amylase signal sequence, which directs the protein to be secreted.
Alpha Amylase
α-Amylase is a protein enzyme that hydrolyses alpha bonds of long alpha-linked polysaccharides, such as glycogen.
ADH1 Terminator
This part is the terminator region from yeast alcohol dehydrogenase gene.
Usage and Biology
This part was proved functional by the 2015 BRHS iGEM Team. Please click the below link to see their results. http://2015.igem.org/Team:BroadRun-NorthernVA/Results
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 8
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1436
Illegal NgoMIV site found at 1451 - 1000COMPATIBLE WITH RFC[1000]