Difference between revisions of "Part:BBa K2065000:Experience"
(→‎Applications of BBa_K2065000)
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===Applications of BBa_K2065000===
 
===Applications of BBa_K2065000===
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This part was used in combination with LargeBiT, which dimerized on our heterodimeric T-14-3-3 scaffold proteins, and together formed the functional luciferase enzyme. The enzyme showed luminescence at 460 nm, thus confirming its working.  
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iGEM TU Eindhoven used this part in combination with CT52-LargeBiT (BBa_K2065007). These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. Thus this system was used as a read out method for T14-3-3 heterodimers.
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===User Reviews===
 
===User Reviews===

Revision as of 11:50, 17 October 2016


Applications of BBa_K2065000

iGEM TU Eindhoven used this part in combination with CT52-LargeBiT (BBa_K2065007). These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. Thus this system was used as a read out method for T14-3-3 heterodimers.

User Reviews

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