Difference between revisions of "Part:BBa K2042014:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We inserted non-coding scar sequences after RBS | + | We inserted non-coding scar sequences after RBS to prevent missing initial codons during translation. |
The operon was integrated in pSEVA224 plasmid in order to produce Polylactic acid in Pseudomonas putida. | The operon was integrated in pSEVA224 plasmid in order to produce Polylactic acid in Pseudomonas putida. | ||
Latest revision as of 22:22, 15 October 2016
RBS + PhaC (sp. MBEL 6-19) + RBS + PCT (CP)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2386
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1073
Illegal NgoMIV site found at 1352
Illegal AgeI site found at 148
Illegal AgeI site found at 461 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2792
Illegal BsaI site found at 3263
Design Notes
We inserted non-coding scar sequences after RBS to prevent missing initial codons during translation. The operon was integrated in pSEVA224 plasmid in order to produce Polylactic acid in Pseudomonas putida.
Source
The RBS were synthesized from the genomic sequence of P.putida, Pct gene were synthetised from genomic sequence of Clostridum propionicum and PhaC gene from Pseudomonas sp. MBEL 6-19