Difference between revisions of "Part:BBa K1951005"
(→Design summary) |
|||
| Line 12: | Line 12: | ||
=== Design summary === | === Design summary === | ||
| − | Starting from the <i>E.coli</i> flagellin sequence ( http://www.uniprot.org/uniprot/P04949 ) we have designed a part to produce this protein in <i>E. coli</i> chassis. | + | Starting from the <i>E.coli</i> flagellin sequence ( http://www.uniprot.org/uniprot/P04949 ) we have designed a part to produce this protein in <i>E. coli</i> chassis [https://parts.igem.org/Part:BBa_K1951008 BBa_K1951008]. |
All forbidden restriction sites have been removed. | All forbidden restriction sites have been removed. | ||
Codons have been optimized for <i> E.coli </i> to allow a maximal transcription level. | Codons have been optimized for <i> E.coli </i> to allow a maximal transcription level. | ||
Revision as of 11:03, 17 October 2016
FliC E.coli coding sequence
This sequence codes for the Flagellin (FliC) protein of Escherichia coli. This protein froms the filament of the bacterial flagellum.
Flagellin is the main protein which polymerizes to form the filaments of bacterial flagella and is necessary for bacterial movement by swimming. The possession of a flagellum allow bacteria to swim towards food sources and so gives them a selective advantage. [1]
It has been demonstrated that Flagellin has the ability to adsorb precious metals such as Platinum, Gold..
Design summary
Starting from the E.coli flagellin sequence ( http://www.uniprot.org/uniprot/P04949 ) we have designed a part to produce this protein in E. coli chassis BBa_K1951008. All forbidden restriction sites have been removed. Codons have been optimized for E.coli to allow a maximal transcription level.
Proof of functionality
This sequence has been tested: placed under the control of a strong promoter with a ribosome binding site the sequence has been validated as functional. For further information, go on the following link : BBa_K1951008 design
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1224
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 301
Illegal AgeI site found at 709 - 1000COMPATIBLE WITH RFC[1000]