Difference between revisions of "Part:BBa K1995010"

Latest revision as of 18:36, 15 October 2016

PH (INP-N-H)

Based on BBa_K1995008 (BH), we added INP-N to the upstream of BH. Using INP-N, we anchored BH out of the outer membrane of E.coli in order to increase the binding efficiency of cupric and mercuric ions.

Ice nucleation protein (INP)

To increase the binding efficiency and detect the concentration of heavy metals, we used INP to anchor BH out of the outer membrane of E. coli.

Ice nucleation protein (INP) is an excretion surface protein. It is widely used for establishing germ surface display system. It contains an internal repeated domain (IRD) and an N-terminal anchoring domain (fig.1). Studies have shown that decrease the time of repetition of IRD can also get a better-off rate of displaying. So we decreased the time of repetition of IRD to reduce pressure of synthesis of E. coli.

Binding heavy metals

We detect the binding efficiency of PH and other parts and get the following chart. (Fig. 5)

Sequence and Features