Difference between revisions of "Part:BBa I712009"

Revision as of 18:37, 15 October 2016

CD4 signal peptide; localizes to plasma membrane

Additional characterization of BBaI712009 by [http://2016.igem.org/Team:LMU-TUM_Munich Munich 2016]:

This part was submitted before the RFC[25] standard was established. Thus this part carries a modified RFC[10] pre- and suffix that changes the reading frame of the suffix in a way that, if it is ligated to a second part, it does not generate a stop codon. Although this approach makes it possible to assemble fusion proteins, the ligation to a second part, which does not carry the modified RFC[10], leads to a frameshift in the second part in a way that it is not functional anymore.

Because signal peptides are such important BioBricks we designed new signal peptide parts such as BBa_K2170215 and BBa_K2170214 to help other iGEM teams assembling fusion proteins together.

Coding region for first 25 amino acids (including start codon) of CD4 receptor which is signal peptide for plasma membrane localization.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 Additional characterization of BBaI712009 by [http://2016.igem.org/Team:LMU-TUM_Munich Munich 2016]:
-This part contains a forbidden PstI site, which is generated by a point mutation in position 1.972. Because this restiction site makes the ligation of a BioBrick in the downstam region of this BioBrick by RFC[25] impossible the PstI site was removed by our team so that the resulting BioBrick [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170150 BBa_K2170150]  matches the RFC[25] standard.
+This part was submitted before the RFC[25] standard was established. Thus this part carries a modified RFC[10] pre- and suffix that changes the reading frame of the suffix in a way that, if it is ligated to a second part, it does not generate a stop codon. Although this approach makes it possible to assemble fusion proteins, the ligation to a second part, which does not carry the modified RFC[10], leads to a frameshift in the second part in a way that it is not functional anymore.
+Because signal peptides are such important BioBricks we designed new signal peptide parts such as [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170215 BBa_K2170215] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170214 BBa_K2170214] to help other iGEM teams assembling fusion proteins together.
-Additionally the BioBrick [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170060 BBa_K2170060] was assembled. This BioBrick contains a poly adenylation site, which was added for improved mRNA stability