Difference between revisions of "Part:BBa K2012007"
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We've also constructed a series of generators with different strength promoter,BBa_K2012013[https://parts.igem.org/Part:BBa_K2012013], BBa_K2012014[https://parts.igem.org/Part:BBa_K2012014],BBa_K2012016[https://parts.igem.org/Part:BBa_K2012016]
 
We've also constructed a series of generators with different strength promoter,BBa_K2012013[https://parts.igem.org/Part:BBa_K2012013], BBa_K2012014[https://parts.igem.org/Part:BBa_K2012014],BBa_K2012016[https://parts.igem.org/Part:BBa_K2012016]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 17:03, 15 October 2016


CheZ fussed with a CG linker and reporter GFP(BBa_E0040)

the whole part contains CheZ fused with GFP,which can show the concentration of CheZ. This coding part do not have its RBS and promoter. Users have to add their preferred RBS and promoter by using biobrick assembly or other suitable assembly. We have proved the function of emitting green light under certain exciting light and the function of improving motility by transform it into a cheZ lacking strain, CL1.  
We've also constructed a series of generators with different strength promoter,BBa_K2012013[1], BBa_K2012014[2],BBa_K2012016[3]
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1301