Difference between revisions of "Part:BBa I712009"
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<partinfo>BBa_I712009 SequenceAndFeatures</partinfo> | <partinfo>BBa_I712009 SequenceAndFeatures</partinfo> | ||
| − | Additional characterization of BBaI712009 by | + | Additional characterization of BBaI712009 by Munich 2016: |
| + | |||
| + | This part was submitted before the RFC[25] standard was established. Thus this part carries a modified RFC[10] pre- and suffix that changes the reading frame of the suffix in a way that, if you ligate it to a second part does not generate a stop codon. Although this approach makes it possible to generate fusion proteins the ligation to a second part, which does not carry the modified RFC[10] leads to a frameshift in the second part. | ||
| + | |||
| + | This is why we designed new signal peptide parts such as [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170215] | ||
Revision as of 17:37, 15 October 2016
CD4 signal peptide; localizes to plasma membrane
Coding region for first 25 amino acids (including start codon) of CD4 receptor which is signal peptide for plasma membrane localization.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Additional characterization of BBaI712009 by Munich 2016:
This part was submitted before the RFC[25] standard was established. Thus this part carries a modified RFC[10] pre- and suffix that changes the reading frame of the suffix in a way that, if you ligate it to a second part does not generate a stop codon. Although this approach makes it possible to generate fusion proteins the ligation to a second part, which does not carry the modified RFC[10] leads to a frameshift in the second part.
This is why we designed new signal peptide parts such as [1]