Difference between revisions of "Part:BBa K2012009"
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Bacteria movement related gene CheZ which fused with a GS linker and GFP in C teminal. We will use this part to charactise the expression of CheZ. These parts are present in plasmid pSB1C3, but there is also a constitutive promoter(J23100-derived)
 
Bacteria movement related gene CheZ which fused with a GS linker and GFP in C teminal. We will use this part to charactise the expression of CheZ. These parts are present in plasmid pSB1C3, but there is also a constitutive promoter(J23100-derived)
 
So, for example, the EcoRI/PstI region of part K2012009 reads:
 
So, for example, the EcoRI/PstI region of part K2012009 reads:
−
[[Biobrick5' XbaI J23100 XbaI '''K2012009''' Biobrick 3' <br>
+
[[----Biobrick5' XbaI J23100 XbaI '''K2012009''' Biobrick 3' <br/>
−
gaattcgcggccgcttctagaGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTtctaga'''sequence part...'''actagtagcggccgctgcag | .]]
+
gaattcgcggccgcttctagaGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTtctaga'''sequence part...'''actagtagcggccgctgcag |----- .]]
 
<html>
 
<html>
  

Revision as of 14:35, 15 October 2016


RBS(J61100) and CheZ with fused with a GS linker and GFP in C teminal

Bacteria movement related gene CheZ which fused with a GS linker and GFP in C teminal. We will use this part to charactise the expression of CheZ. These parts are present in plasmid pSB1C3, but there is also a constitutive promoter(J23100-derived) So, for example, the EcoRI/PstI region of part K2012009 reads: [[----Biobrick5' XbaI J23100 XbaI K2012009 Biobrick 3'
gaattcgcggccgcttctagaGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTtctagasequence part...actagtagcggccgctgcag |----- .]]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1321