Difference between revisions of "Part:BBa K1934061:Experience"
 
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===Applications of BBa_K1934061===
 
===Applications of BBa_K1934061===
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This part has been transferred into e. coli NM522 for expression. After 6 hours at 37° IPTG has been added to the final concentration of 1 mM. The expression culture was grown overnight at 30°. We the used instructions provided on the NiNTA columns kit from Quigen to purify the protein.  
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This part has been transferred into <i>E. coli</i> NM522 for expression. After 6 hours at 37°C, IPTG was added to a final concentration of 1mM. The expression culture was grown overnight at 30°C. We then used instructions provided on the Ni-NTA columns kit from Qiagen to purify the protein.  
 
===User Reviews===
 
===User Reviews===
 
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Latest revision as of 11:55, 19 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1934061

This part has been transferred into E. coli NM522 for expression. After 6 hours at 37°C, IPTG was added to a final concentration of 1mM. The expression culture was grown overnight at 30°C. We then used instructions provided on the Ni-NTA columns kit from Qiagen to purify the protein.

User Reviews

UNIQ9238cb57bbe16eb8-partinfo-00000000-QINU UNIQ9238cb57bbe16eb8-partinfo-00000001-QINU