Difference between revisions of "Part:BBa K2110105"
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<partinfo>BBa_K2110105 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2110105 SequenceAndFeatures</partinfo> | ||
===Results=== | ===Results=== | ||
| − | We detect the absorption peak of culture medium after culturing with PET film added for | + | We detect the absorption peak of culture medium after culturing with PET film added for 5d. The absorption degree-wavelength curve is as follows. |
https://static.igem.org/mediawiki/parts/thumb/7/7a/T--Tianjin--partdataI208V.png/800px-T--Tianjin--partdataI208V.png | https://static.igem.org/mediawiki/parts/thumb/7/7a/T--Tianjin--partdataI208V.png/800px-T--Tianjin--partdataI208V.png | ||
Revision as of 12:19, 15 October 2016
PETase site-directed mutant I208V
This is one of the site-directed mutant of the PETase gene, we change the 208th amino acid from I to V. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 79
Illegal BglII site found at 289 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Results
We detect the absorption peak of culture medium after culturing with PET film added for 5d. The absorption degree-wavelength curve is as follows.
We used the relative fluorescence units to represent the expression level of enzymes.
Finally we got the relative activities of enzymes.
