Difference between revisions of "Part:BBa K2110101"
(→‎Results)
Line 15: Line 15:
 
We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows.
 
We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows.
 
https://static.igem.org/mediawiki/parts/thumb/7/7a/T--Tianjin--partdataI208V.png/800px-T--Tianjin--partdataI208V.png
 
https://static.igem.org/mediawiki/parts/thumb/7/7a/T--Tianjin--partdataI208V.png/800px-T--Tianjin--partdataI208V.png
−
 
 
We used the relative fluorescence units to represent the expression level of enzymes.  
 
We used the relative fluorescence units to represent the expression level of enzymes.  
 
https://static.igem.org/mediawiki/2016/1/1d/T--Tianjin--part-I208Vex.png
 
https://static.igem.org/mediawiki/2016/1/1d/T--Tianjin--part-I208Vex.png
−
 
 
Finally we got the relative activities of enzymes.  
 
Finally we got the relative activities of enzymes.  
 
https://static.igem.org/mediawiki/2016/2/2f/T--Tianjin--part-I208Vbar.png
 
https://static.igem.org/mediawiki/2016/2/2f/T--Tianjin--part-I208Vbar.png
−
 
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2110101 parameters</partinfo>
 
<partinfo>BBa_K2110101 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Revision as of 10:22, 15 October 2016


PETase site-directed mutant S207T

This is one of the site-directed mutant of the PETase gene, we change the 208th amino acid from I to V. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant. Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 79
    Illegal BglII site found at 289
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Results

We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows. 800px-T--Tianjin--partdataI208V.png We used the relative fluorescence units to represent the expression level of enzymes. T--Tianjin--part-I208Vex.png Finally we got the relative activities of enzymes. T--Tianjin--part-I208Vbar.png