Difference between revisions of "Part:BBa K2113005"
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<br>The instability index (II) is computed to be 163.60
 
<br>The instability index (II) is computed to be 163.60
 
<br>This classifies the protein as unstable.
 
<br>This classifies the protein as unstable.
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<br>Grand average of hydropathicity (GRAVY): -1.678
 
<br>Grand average of hydropathicity (GRAVY): -1.678
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===References===
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<br>1. Ressource Parisienne en Bioinformatique Structurale.(Mobyl@RPBS)
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<br>2. ExPASy PeptideCutter
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<br>3. ExPASy ProtParam

Revision as of 04:13, 15 October 2016


AMP with glycine

This part contains the anti-microbial peptides (AMP) with consecutive sequences of arginine and tryptophan.These peptides have antimicrobial activity against wide range of microbes. In order to increase the shelf life and stability of the peptide glycine residues are added at the N terminal(GGWRWRWR).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Mobyl@RPBS

3D structural view

MGG.gif


ProtParam Results


Number of amino acids: 9
Molecular weight: 1290.51
Theoretical pI: 12.30
Total number of negatively charged residues (Asp + Glu): 0
Total number of positively charged residues (Arg + Lys): 3


Atomic composition:


Carbon (C) 60
Hydrogen (H) 83
Nitrogen (N) 21
Oxygen (O) 10
Sulfur (S) 1


Formula: C60H83N21O10S1
Total number of atoms: 175


Extinction coefficients:


Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.


Ext. coefficient: 16500
Abs 0.1% (=1 g/l): 12.786


Estimated half-life:


The N-terminal of the sequence considered is M (Met).


The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).


Instability index:


The instability index (II) is computed to be 163.60
This classifies the protein as unstable.




Aliphatic index: 0.00


Grand average of hydropathicity (GRAVY): -1.678



References


1. Ressource Parisienne en Bioinformatique Structurale.(Mobyl@RPBS)
2. ExPASy PeptideCutter
3. ExPASy ProtParam