Difference between revisions of "Part:BBa K2100021"
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<partinfo>BBa_K2100021 short</partinfo>
 
<partinfo>BBa_K2100021 short</partinfo>
  
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This entry vector contains the EGSH promoter. pEGSH is part of the ecdysone inducible expression system. The synthetic receptor fusion VgEcR forms a complex with RXR which binds as a heterodimer to the E/GRE recognition sequences on the EGSH promoter, transcribing the gene of choice downstream of the promoter. This system can be induced with the small molecule PonA, and is otherwise tightly controlled by corepressors.  
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This entry vector contains the EGSH promoter. pEGSH is part of the ecdysone inducible expression system. The synthetic receptor fusion VgEcR forms a complex with RXR which binds as a heterodimer to the E/GRE recognition sequences on the EGSH promoter, transcribing the gene of choice downstream of the promoter. This system can be induced with the small molecule PonA, and is otherwise tightly controlled by corepressors.[1]
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[1] https://www.agilent.com/cs/library/usermanuals/Public/217564.pdf
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 02:12, 15 October 2016


pENTR EGSH

This entry vector contains the EGSH promoter. pEGSH is part of the ecdysone inducible expression system. The synthetic receptor fusion VgEcR forms a complex with RXR which binds as a heterodimer to the E/GRE recognition sequences on the EGSH promoter, transcribing the gene of choice downstream of the promoter. This system can be induced with the small molecule PonA, and is otherwise tightly controlled by corepressors.[1]

[1] https://www.agilent.com/cs/library/usermanuals/Public/217564.pdf

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 671
    Illegal PstI site found at 485
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 671
    Illegal PstI site found at 485
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 671
    Illegal BglII site found at 115
    Illegal BamHI site found at 663
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 671
    Illegal PstI site found at 485
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 671
    Illegal PstI site found at 485
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 646