Difference between revisions of "Part:BBa K2070023"
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Ribosomes cannot bind to the Toehold RBS under normal conditions. Therefore, proteins cannot be made because mRNAs cannot be translated even when the gene is transcribed. For a ribosome to bind to a toehold RBS, the existence of a specific short RNA named trigger RNA is  necessary.  
 
Ribosomes cannot bind to the Toehold RBS under normal conditions. Therefore, proteins cannot be made because mRNAs cannot be translated even when the gene is transcribed. For a ribosome to bind to a toehold RBS, the existence of a specific short RNA named trigger RNA is  necessary.  
  
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[[File:T--UT Tokyo--K2070016(1).png|200px|thumb|left]]  
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[[File:T--UT Tokyo--K2070016(1).png]  
  
  

Revision as of 02:13, 15 October 2016


Constitutive ecf16 generator

This part is used to constitutively generate the trigger required for the activation of the Toehold rbs in part BBa_K2070018 to generate GFP. The trigger and toehold together form the Toehold System.

Detailed Explanation of the Toehold System

Ribosomes cannot bind to the Toehold RBS under normal conditions. Therefore, proteins cannot be made because mRNAs cannot be translated even when the gene is transcribed. For a ribosome to bind to a toehold RBS, the existence of a specific short RNA named trigger RNA is necessary.

[[File:T--UT Tokyo--K2070016(1).png]


Reference

Green, A., Silver, P., Collins, J., & Yin, P. (2014, 11). Toehold Switches: De-Novo-Designed Regulators of Gene Expression. Cell, 159(4), 925-939. doi:10.1016/j.cell.2014.10.002


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 239
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 434
  • 1000
    COMPATIBLE WITH RFC[1000]